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Isolere og Imaging Live, Intakt Pacemaker Regioner af Mouse Renal Bækken ved Vibratome Sektion
JoVE Journal
Biology
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JoVE Journal Biology
Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Isolere og Imaging Live, Intakt Pacemaker Regioner af Mouse Renal Bækken ved Vibratome Sektion

DOI:
10.3791/62040-v

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07:38 min

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April 30, 2021

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, ,
1Department of Physiology & Cell Biology,University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology,University of California School of Medicine, 3Department of Life & Health Sciences,Dundalk Institute of Technology

Chapters

  • 00:05Introduction
  • 00:44Preparation and Dissection of Kidney and Calibration of Vibratome Instrument
  • 02:32Vibratome Sectioning and Kidney Slice Ca2+ Image Acquisition
  • 04:06Kidney Slice Ca2+ Image Acquisition
  • 05:14Results: Imaging of Spontaneous Calcium Ion Transient in Vibratome Sections of Whole Kidneys
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at isolere intakt pacemaker regioner af musen nyre bækken ved hjælp af vibratome sektionsopderinger. Disse sektioner kan derefter bruges til in situ Ca2 + billeddannelse til at belyse Ca2 + forbigående egenskaber pacemaker celler og andre interstitielle celler i vibratome skiver.

Tags

Pacemaker RegionsRenal PelvisVibratome SectioningIntact PreparationSmooth MuscleKidneyUreterCalcium Ion ImagingDissectionRazor BladeKRB Solution

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