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Journal / Biology / Isolere og Imaging Live, Intakt Pacemaker…
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Isolere og Imaging Live, Intakt Pacemaker Regioner af Mouse Renal Bækken ved Vibratome Sektion
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Isolere og Imaging Live, Intakt Pacemaker Regioner af Mouse Renal Bækken ved Vibratome Sektion

Article DOI: 10.3791/62040-v • 07:38 min • April 30th, 2021
April 30th, 2021 •
1,2, 1, 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

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Målet med denne protokol er at isolere intakt pacemaker regioner af musen nyre bækken ved hjælp af vibratome sektionsopderinger. Disse sektioner kan derefter bruges til in situ Ca2 + billeddannelse til at belyse Ca2 + forbigående egenskaber pacemaker celler og andre interstitielle celler i vibratome skiver.

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Biologi Problem 170 Nyre bækken pacemaker interstitiel celle glat muskel urinveje Ca2 + billeddannelse urogenitale nyre PDGFRα Ano1
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