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Journal / Biology / Isolere og imaging Live, intakt Pacemaker…
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Isolere og imaging Live, intakt Pacemaker regioner av mus Renal Pelvis av Vibratome seksjonering
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Isolere og imaging Live, intakt Pacemaker regioner av mus Renal Pelvis av Vibratome seksjonering

Article DOI: 10.3791/62040-v • 07:38 min • April 30th, 2021
April 30th, 2021 •
1,2, 1, 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

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Målet med denne protokollen er å isolere intakte pacemaker regioner av musen nyre bekken ved hjelp av vibratome seksjonering. Disse seksjonene kan deretter brukes til in situ Ca2+ avbildning for å belyse Ca2+ forbigående egenskaper til pacemakerceller og andre interstitielle celler i vibratomskiver.

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Biologi Utgave 170 Nyrebekken pacemaker interstitiell celle glatt muskel urinveier Ca2+ avbildning urogenitale nyre PDGFRα Ano1
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