Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited <em>Helicoverpa Armigera</em> (H&#252;bner)

Dong Ai; Bing Wang; Zhennan Fan; Yuyao Fu; Caihong Yu; Guirong Wang

doi:10.3791/62068

Journal

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Behavior

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Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited <em>Helicoverpa Armigera</em> (Hübner)

JoVE Journal
Behavior

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JoVE Journal Behavior

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

4,435 Views

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06:37 min

•

July 01, 2021

DOI:

10.3791/62068-v

DOI:

10.3791/62068-v

•

06:37 min

July 01, 2021

•

4398 Views

Dong Ai^1,2, Bing Wang², Zhennan Fan¹, Yuyao Fu¹, Caihong Yu¹, Guirong Wang^2,3

¹School of Chemical and Environmental Engineering,China University of Mining and Technology ²State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences ³Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences

Summary

Presented here is a protocol of Helicoverpa armigera (Hübner) embryo microinjection and knockout mutant identification created by CRISPR/Cas9 genome editing. Mutant insects enable further research of gene function and interaction among different genes in vivo.