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Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells
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JoVE Journal Neuroscience
Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

DOI:
10.3791/62104-v

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06:47 min

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January 21, 2021

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, ,
1Department of Physiology, College of Medicine,University of Kentucky, 2Universidad Nacional de Colombia

Chapters

  • 00:05Introduction
  • 00:42Nanopipette Testing
  • 01:36Testing Imaging Resolution with Calibration Standards for Atomic Force Microscopy (AFM)
  • 03:29Auditory Hair Cell Imaging
  • 04:51Results: Representative HPICM Stereocilia Bundle Imaging
  • 06:11Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.

Tags

Keywords: StereociliaHair CellsNanoscale ResolutionLive Cell ImagingHPICMNanopipettesCalibration StandardZ ApproachSurface Topography

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