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JoVE Journal
Genetics

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Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
 

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Article DOI: 10.3791/62146-v 09:38 min February 2nd, 2021
February 2nd, 2021

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Summary

The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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Tags

Site-directed Integration ΦC31-mediated Integration Cassette Exchange Anopheles Vectors Malaria Mosquito Genes Transgene Insertion Phenotypes Positional Effect AttB Donor Plasmids AttB Recombination Sites Transgene Cargo AttB Tagged Donor Plasmid Helper Plasmid Integrase DNA Precipitation
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