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Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
Site-Directed <em>φC31</em>-Mediated Integration and Cassette Exchange in <em>Anopheles</em> Vectors of Malaria
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

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09:38 min

February 02, 2021

DOI:

09:38 min
February 02, 2021

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Summary

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The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.

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