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Journal / Neuroscience / ROS Live Cell Imaging under neuronal utveckling
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ROS Live Cell Imaging under neuronal utveckling
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ROS Live Cell Imaging under neuronal utveckling

Article DOI: 10.3791/62165-v • 09:25 min • February 9th, 2021
February 9th, 2021 •
1,2, 1,2, 1,2,3
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Integrative Neuroscience, Purdue University, 3Bindley Bioscience Center, Purdue University

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Detta protokoll beskriver användningen av en genetiskt kodad väteperoxid (H2O2)-biosensori odlade zebrafisk nervceller och larver för att bedöma de fysiologiska signalrollerna för H2O2 under nervsystemet utveckling. Det kan appliceras på olika celltyper och modifieras med experimentella behandlingar för att studera reaktiva syrearter (ROS) i allmän utveckling.

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Neurovetenskap Nummer 168 ROS väteperoxid roGFP2-Orp1 biosensor RGC zebrafisk neuronal utveckling
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