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Immunology and Infection
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Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

DOI:
10.3791/62187-v

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09:26 min

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January 22, 2021

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, , , , ,
1School of Life Science,University of Warwick, 2Institute of Microbiology and Infection,University of Birmingham, 3Warwick Antimicrobial Screening Facility, School of Life Science,University of Warwick

Chapters

  • 00:04Introduction
  • 00:52Dissection and Infection of Ex Vivo Pig Lung (EVPL) Tissue
  • 04:45Determination of Antibiotic Efficacy
  • 06:39Results: Screening Bacterial Isolates for Antibiotic Susceptibility with the EVPL Model
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

This workflow can be used to perform antibiotic susceptibility testing using an established ex vivo model of bacterial biofilm in the lungs of individuals with cystic fibrosis. Use of this model could enhance the clinical validity of MBEC (minimal biofilm eradication concentration) assays.

Tags

Keywords: Antibiotic EfficacyPseudomonas AeruginosaStaphylococcus AureusBiofilmsCystic Fibrosis LungEx Vivo ModelBronchioleDMEM RPMI 1640Antibiotic Susceptibility Testing

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