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In vivo Measurement of Knee Extensor Muscle Function in Mice
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In vivo Measurement of Knee Extensor Muscle Function in Mice

In vivo Measurement of Knee Extensor Muscle Function in Mice

DOI:
10.3791/62211-v

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08:29 min

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March 04, 2021

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, , , , ,
1Department of Athletic Training and Clinical Nutrition,University of Kentucky, 2Center for Muscle Biology,University of Kentucky, 3Department of Physical Therapy,East Carolina University, 4Kinesiology and Health Promotion Graduate Program,University of Kentucky, 5Biomotion Lab, College of Health Sciences,University of Kentucky, 6Aurora Scientific, 7Department of Physical Therapy, College of Health Sciences,University of Kentucky

Chapters

  • 00:04Introduction
  • 01:00Equipment Setup
  • 01:33Software Setup
  • 02:29Mouse Setup
  • 03:24Electrode Placement
  • 04:18Determination of Optimal Current
  • 05:00Torque-Frequency Experiment to Determine Peak Isometric Tetanic Torque
  • 06:10Results: In Vivo Measurement of Knee Extensor Muscle Function in Mice
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

Quantification of knee extensor maximal strength is imperative to understand functional adaptations to aging, disease, injury, and rehabilitation. We present a novel method to repeatedly measure in vivo knee extension isometric peak tetanic torque.

Tags

Keywords: Knee ExtensorMuscle FunctionMiceIsometric Peak Tetanic TorqueNoninvasive AssessmentQuadricep StrengthPre-clinical ModelsOsteoarthritisKnee InjuryElectrodesStimulationTwitchTorque FrequencyAnesthesiaSedationShavingClamp

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