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Journal / Immunology and Infection / 小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究…
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Immunology and Infection

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JoVE Journal Immunology and Infection
小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究
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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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我们描述了一种快速而强大的方案，以从小鼠脾脏中富集不变的自然杀伤T（iNKT）细胞，并在体外将其扩增至适合体外和体内研究的数量。

Tags

免疫学和感染，第168期，iNKT细胞，扩增，小鼠，Vα14，CD1d，抗CD3 / CD28微球，活化，淋巴细胞
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