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Journal
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Immunology and Infection
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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究
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Immunology and Infection
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Immunology and Infection
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
Please note that all translations are automatically generated.
Click here for the English version.
小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究
DOI:
10.3791/62214-v
•
08:37 min
•
February 15, 2021
•
Gloria Delfanti
,
Alessandra Perini*
1,2
,
Elisa Zappa*
1,2
,
Maya Fedeli
2
1
Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases
,
IRCCS San Raffaele Scientific Institute
,
2
Vita-Salute San Raffaele University
Chapters
00:04
Introduction
00:39
Spleen Processing
01:29
T Cell Enrichment
03:27
iNKT Cell Enrichment
05:21
iNKT Cell Activation and Expansion
06:35
Results: Enrichment, Expansion, and Characterization of iNKT Cells
07:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种快速而强大的方案,以从小鼠脾脏中富集不变的自然杀伤T(iNKT)细胞,并在体外将其扩增至适合体外和体内研究的数量。
Tags
Mouse Invariant Natural Killer T Cells
Purification
Expansion
In Vitro
In Vivo
Cell Isolation
Cell Enrichment
MACS
Cell Staining
Cell Separation
Cell Culture
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