Name: Robot-Assisted Kidney Transplantation (Video) | JoVE Journal
Uploaded: 2021-07-19T13:00:00.000+00:00
Duration: 7 min 30 s
Description: Learn robot-assisted kidney transplantation using a lateral allograft positioning technique that avoids flipping. This protocol demonstrates vascular anastomosis with reduced risk of vessel torsion for improved surgical outcomes.

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5 minutes

We thank Dr. Joon Seo Lim from the Scientific Publications Team at Asan Medical Center for his editorial assistance in preparing this manuscript.

This study got approval from the Institutional Review Board of Asan Medical Center (IRB number: 2021-0101).
1. Pretransplant preparation
<ol>
	<li>Patient selection
	<ol>
		<li>Include patients with end-stage renal disease who require kidney transplantation. 
		NOTE: RAKT may not be considered if a recipient is younger than eighteen years old.</li>
		<li>Exclude those with any kind of untreated malignancy or active infection.</li>
		<li>Ensure that the recipient is suitable for surgery with respect to cardiac and pulmonary function and appropriate for a minimally invasive approach.</li>
		<li>Do not consider RAKT if a patient has a history of major abdominal surgery or severe intraperitoneal adhesion. In addition, do not consider RAKT and recommend open kidney transplantation if there is severe calcification in the iliac arteries on computerized tomography.</li>
	</ol>
	</li>
	<li>Patient preparation
	<ol>
		<li>Begin the standard presurgical preparation. Administer laxative suppository tablets for bowel preparation. Ensure that the patient does not ingest anything orally from midnight of the day of the operation. Administer prophylactic first-generation cephalosporin just before a skin incision.</li>
		<li>Provide the maintenance immunosuppressants (e.g., calcineurin inhibitors, methylprednisolone, mycophenolate mofetil) from two days (conventional cases) or seven days (ABO-incompatible or human leukocyte antigen-incompatible cases) before the transplantation according to the protocol of the respective center.</li>
		<li>Prepare the induction immunosuppressants (i.e., anti-thymocyte globulin or basiliximab) that will be administered during the RAKT.</li>
	</ol>
	</li>
	<li>Equipment
	<ol>
		<li>Ensure the availability of a robotic system.</li>
		<li>Ensure the availability of standard laparoscopic equipment and robotic instruments (see the Table of Materials).</li>
		<li>Ensure the availability of 6/0 or 7/0 polytetrafluoroethylene (ePTFE) sutures for artery and vein anastomosis.</li>
		<li>Ensure the availability of 6/0 polydioxanone suture and 3/0 polyglactin adsorbable suture for neocystoureterostomy.</li>
		<li>Ensure the availability of a double-J stent.</li>
	</ol>
	</li>
</ol>
2. Surgical preparation
<ol>
	<li>Anesthesia
	<ol>
		<li>Evaluate the operative risk according to the American Society of Anesthesiologists&#39; classification of Physical Health.</li>
		<li>Induce general anesthesia and use rocuronium bromide as a muscle-relaxant.</li>
		<li>Insert a central venous line and an arterial line.</li>
		<li>Insert a foley catheter and fill the bladder with normal saline. Keep the foley catheter clamped until ureteroneocystostomy is performed.</li>
		<li>Perform arterial blood gas analyses at 1 h intervals during the transplantation.</li>
		<li>Reverse the anesthesia with sugammadex (2 mg/kg, intravenous) at the end of the surgery.</li>
	</ol>
	</li>
	<li>Operation field 
	NOTE: A schematic arrangement map of the operating room is shown in Figure 1.
	<ol>
		<li>Have the operator perform procedures from the robotic console.</li>
		<li>Have the first assistant stand on the left side of the patient. 
		NOTE: The first assistant will be in charge of performing irrigation and suction, supplying sutures and bulldog clamps, and helping with retraction.</li>
		<li>Have the second assistant stand on the right side of the patient&#39;s hip to exchange robotic instruments and help the first assistant.</li>
		<li>Have a scrub nurse stand on the left side of the patient&#39;s left leg.</li>
		<li>Place the patient in the left lateral decubitus position with the legs parted and the Trendelenburg position (20&#176;-30&#176;). Dock the robot between the legs.</li>
	</ol>
	</li>
	<li>Preparation of the kidney allograft (Figure 2)
	<ol>
		<li>Ensure that cold ischemia is started immediately after recovering the kidney from the living donor. Remove the perinephric fat tissue and perform meticulous ligation of the lymphatics around the hilum of kidney allograft on a back table.</li>
		<li>Measure the weight and size of the kidney allograft.</li>
		<li>Consider arterial reconstruction if there are multiple renal arteries such as side-to-side anastomosis, end-to-side anastomosis of the polar artery into the main renal artery, and polar artery anastomosis to the inferior epigastric artery.</li>
		<li>Consider venous extension with a gonadal vein of the recipient or an iliac vein of the deceased donor.</li>
		<li>Insert a 4.8-French, 12 cm double-J stent in the ureter using a guide-wire.</li>
		<li>Wrap the kidney allograft in an ice-packed gauze.</li>
	</ol>
	</li>
</ol>
3. Positioning of the robotic and gel ports (&#160;Figure 3)
<ol>
	<li>Establish and maintain a pneumoperitoneum at approximately 10 mmHg. 
	NOTE: Trocar positioning is for right-sided kidney transplantation.</li>
	<li>Introduce the 12 mm or 8 mm robotic camera port just above the umbilicus. 
	NOTE: The camera port should be placed at about 10-15 cm from the nearest boundary of the target anatomy.</li>
	<li>Place the 8 mm robotic port for Arm II on the right lateral side at 8-9 cm away from the camera port.</li>
	<li>Place another 8 mm robotic port for Arm III along the line between the umbilicus and anterior superior iliac spine at a distance of approximately 8-9 cm from the umbilicus.</li>
	<li>Place the other 8 mm robotic port for Arm IV at approximately 8-9 cm laterally to the port for Arm III. 
	NOTE: Ensure a distance of 2 cm between the ports and bony prominences.</li>
	<li>Place the gel port (6 cm Pfannenstiel incision) on the right suprapubic area (the target anatomy). Make two or three ports on the gel port for the first and second assistants.</li>
</ol>
4. Intraabdominal dissection and insertion of the kidney allograft (Video 1)
<ol>
	<li>Incise the peritoneum along the right paracolic gutter to make a pouch for the kidney allograft with monopolar curved scissors (Arm II), fenestrated bipolar forceps (Arm III), and Prograsp forceps (Arm IV) (see the Table of Materials).</li>
	<li>Dissect the right external iliac vessels along their entire length. Encircle each vessel with a vessel loop.</li>
	<li>Dissect the bladder for ureteroneocystostomy on the right corner of the bladder and separate it from the peritoneal incision for the kidney allograft.</li>
	<li>After opening a cap of the gel port, insert slushed ice followed by the kidney allograft wrapped in the ice-packed gauze through the 6 cm Pfannenstiel incision.</li>
	<li>Place the allograft on the peritoneal pouch lateral to the iliac vessels on the right side.</li>
</ol>
5. Vascular anastomosis and reperfusion (Video 1)
<ol>
	<li>Keep the allograft as cold as possible with either slushed ice or cold normal saline.</li>
	<li>Clamp the right external iliac vein distal and proximal to the anastomosis site with Bulldog clamps, manipulated by Prograsp forceps (Arm IV).</li>
	<li>Make a venotomy with Potts scissors in a linear or oblique fashion, considering the diameter of the renal vein.</li>
	<li>Anastomose the allograft renal vein to the right external iliac vein in an end-to-side continuous manner using a 6/0 ePTFE suture. Make a knot at the caudal end of veins, and suture the posterior wall intraluminally in a continuous manner. Afterwards, suture the anterior wall in a continuous manner. 
	NOTE: The anastomosis is performed with a large needle driver on Arm II and black diamond microforceps or Maryland forceps on Arm III for right-handed surgeons.</li>
	<li>Flush the lumen with heparinized normal saline (5 IU/mL) just before knotting the anastomosis using a silastic tube through the gel port.</li>
	<li>Clamp the allograft renal vein with a Bulldog clamp.</li>
	<li>Declamp the right external iliac vein.</li>
	<li>Clamp the right external iliac artery proximal and distal to the anastomosis site with Bulldog clamps.</li>
	<li>Make an arteriotomy with Potts scissors. Create a round hole with Potts scissors and without an arterial punch.</li>
	<li>Using the same method as vein anastomosis, anastomose the allograft renal artery to the right external iliac artery in an end-to-side continuous manner using a 6/0 ePTFE suture.</li>
	<li>Flush the lumen with heparinized normal saline just before knotting the anastomosis using a silastic tube through the gel port.</li>
	<li>Clamp the allograft renal artery with a Bulldog clamp.</li>
	<li>Declamp the right external iliac artery.</li>
	<li>Declamp the allograft renal vein and artery if there is no evident bleeding at the anastomosis sites.</li>
	<li>Remove the ice-packed gauze.</li>
	<li>Apply warm normal saline on the allograft with an irrigation tube through the gel port.</li>
</ol>
6. Ureteroneocystostomy and peritoneal covering (Video 1)
<ol>
	<li>Perform ureteroneocystostomy according to the Lich-Gregoir technique11.</li>
	<li>Put the distal end of the double-J stent into the bladder.</li>
	<li>Starting at the posterior corner, perform a continuous suture using a 6/0 polydioxanone suture and make a knot at the anterior corner. Then, perform a continuous suture from the anterior corner to the posterior corner.</li>
	<li>From the anterior corner to the posterior corner, close the detrusor muscle antireflux tunnel in an interrupted manner using a 4/0 polyglactin multifilament absorbable suture.</li>
	<li>Cover the kidney allograft with the incised peritoneum along the right paracolic gutter intermittently using polymer locking clips.</li>
</ol>
7. Wound closure
<ol>
	<li>Insert a closed-suction drain through the 8 mm robotic port for Arm II on the right lateral side and put the drain around the kidney allograft.</li>
	<li>Deflate the pneumoperitoneum by opening the gel port.</li>
	<li>Close the gel port and the camera port incisions layer by layer (peritoneum, muscles, subcutaneous layer, and skin). Close the 8 mm robotic port incisions only at the level of the subcutaneous layer and skin.</li>
</ol>

<ol><li>Wolfe, R. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+mortality+in+all+patients+on+dialysis%2C+patients+on+dialysis+awaiting+transplantation%2C+and+recipients+of+a+first+cadaveric+transplant%5BTitle%5D)+AND+%22New+England+Journal+of+Medicine%22%5BJournal%5D)">Comparison of mortality in all patients on dialysis, patients on dialysis awaiting transplantation, and recipients of a first cadaveric transplant.</a> New England Journal of Medicine. 341 (23), 1725-1730 (1999).</li>
<li>Hoznek, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+assisted+kidney+transplantation%3A+an+initial+experience%5BTitle%5D)+AND+%22Journal+of+Urology%22%5BJournal%5D)">Robotic assisted kidney transplantation: an initial experience.</a> Journal of Urology. 167 (4), 1604-1606 (2002).</li>
<li>Breda, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic-assisted+kidney+transplantation%3A+our+first+case%5BTitle%5D)+AND+%22World+Journal+of+Urology%22%5BJournal%5D)">Robotic-assisted kidney transplantation: our first case.</a> World Journal of Urology. 34 (3), 443-447 (2016).</li>
<li>Menon, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+kidney+transplantation+with+regional+hypothermia%3A+evolution+of+a+novel+procedure+utilizing+the+IDEAL+guidelines+%28IDEAL+phase+0+and+1%29%5BTitle%5D)+AND+%22European+Urology%22%5BJournal%5D)">Robotic kidney transplantation with regional hypothermia: evolution of a novel procedure utilizing the IDEAL guidelines (IDEAL phase 0 and 1).</a> European Urology. 65 (5), 1001-1009 (2014).</li>
<li>Tzvetanov, I., D'Amico, G., Benedetti, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic-assisted+kidney+transplantation%3A+our+experience+and+literature+review%5BTitle%5D)+AND+%22Current+Transplantation+Reports%22%5BJournal%5D)">Robotic-assisted kidney transplantation: our experience and literature review.</a> Current Transplantation Reports. 2 (2), 122-126 (2015).</li>
<li>Giulianotti, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+transabdominal+kidney+transplantation+in+a+morbidly+obese+patient%5BTitle%5D)+AND+%22American+Journal+of+Transplantation%22%5BJournal%5D)">Robotic transabdominal kidney transplantation in a morbidly obese patient.</a> American Journal of Transplantation. 10 (6), 1478-1482 (2010).</li>
<li>Oberholzer, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Minimally+invasive+robotic+kidney+transplantation+for+obese+patients+previously+denied+access+to+transplantation%5BTitle%5D)+AND+%22American+Journal+of+Transplantation%22%5BJournal%5D)">Minimally invasive robotic kidney transplantation for obese patients previously denied access to transplantation.</a> American Journal of Transplantation. 13 (3), 721-728 (2013).</li>
<li>Tzvetanov, I. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+kidney+transplantation+in+the+obese+patient%3A+10-year+experience+from+a+single+center%5BTitle%5D)+AND+%22American+Journal+of+Transplantation%22%5BJournal%5D)">Robotic kidney transplantation in the obese patient: 10-year experience from a single center.</a> American Journal of Transplantation. 20 (2), 430-440 (2020).</li>
<li>Garcia-Roca, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single+center+experience+with+robotic+kidney+transplantation+for+recipients+with+BMI+of+40+kg%2Fm2+or+greater%3A+a+comparison+with+the+UNOS+registry%5BTitle%5D)+AND+%22Transplantation%22%5BJournal%5D)">Single center experience with robotic kidney transplantation for recipients with BMI of 40 kg/m2 or greater: a comparison with the UNOS registry.</a> Transplantation. 101 (1), 191-196 (2017).</li>
<li>Gallioli, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Learning+curve+in+robot-assisted+kidney+transplantation%3A+results+from+the+European+Robotic+Urological+Society+Working+Group%5BTitle%5D)+AND+%22European+Urology%22%5BJournal%5D)">Learning curve in robot-assisted kidney transplantation: results from the European Robotic Urological Society Working Group.</a> European Urology. 78 (2), 239-247 (2020).</li>
<li>Alberts, V. P., Idu, M. M., Legemate, D. A., Laguna Pes, M. P., Minnee, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ureterovesical+anastomotic+techniques+for+kidney+transplantation%3A+a+systematic+review+and+meta-analysis%5BTitle%5D)+AND+%22Transplant+International%22%5BJournal%5D)">Ureterovesical anastomotic techniques for kidney transplantation: a systematic review and meta-analysis.</a> Transplant International. 27 (6), 593-605 (2014).</li>
<li>Modi, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Retroperitoneoscopic+living-donor+nephrectomy+and+laparoscopic+kidney+transplantation%3A+experience+of+initial+72+cases%5BTitle%5D)+AND+%22Transplantation%22%5BJournal%5D)">Retroperitoneoscopic living-donor nephrectomy and laparoscopic kidney transplantation: experience of initial 72 cases.</a> Transplantation. 95 (1), 100-105 (2013).</li>
<li>Oberholzer, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Minimally+invasive+robotic+kidney+transplantation+for+obese+patients+previously+denied+access+to+transplantation%5BTitle%5D)+AND+%22American+Journal+of+Transplantation%22%5BJournal%5D)">Minimally invasive robotic kidney transplantation for obese patients previously denied access to transplantation.</a> American Journal of Transplantation. 13 (3), 721-728 (2013).</li>
<li>Menon, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+kidney+transplantation+with+regional+hypothermia%3A+a+step-by-step+description+of+the+Vattikuti+Urology+Institute-Medanta+technique+%28IDEAL+phase+2a%29%5BTitle%5D)+AND+%22European+Urology%22%5BJournal%5D)">Robotic kidney transplantation with regional hypothermia: a step-by-step description of the Vattikuti Urology Institute-Medanta technique (IDEAL phase 2a).</a> European Urology. 65 (5), 991-1000 (2014).</li>
<li>Tsai, M. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robot-assisted+renal+transplantation+in+the+retroperitoneum%5BTitle%5D)+AND+%22Transplant+International%22%5BJournal%5D)">Robot-assisted renal transplantation in the retroperitoneum.</a> Transplant International. 27 (5), 452-457 (2014).</li>
<li>Sood, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Minimally+invasive+kidney+transplantation%3A+perioperative+considerations+and+key+6-month+outcomes%5BTitle%5D)+AND+%22Transplantation%22%5BJournal%5D)">Minimally invasive kidney transplantation: perioperative considerations and key 6-month outcomes.</a> Transplantation. 99 (2), 316-323 (2015).</li>
<li>Modi, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Laparoscopic+transplantation+following+transvaginal+insertion+of+the+kidney%3A+description+of+technique+and+outcome%5BTitle%5D)+AND+%22American+Journal+of+Transplantation%22%5BJournal%5D)">Laparoscopic transplantation following transvaginal insertion of the kidney: description of technique and outcome.</a> American Journal of Transplantation. 15 (7), 1915-1922 (2015).</li>
<li>Wagenaar, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Minimally+invasive%2C+laparoscopic%2C+and+robotic-assisted+techniques+versus+open+techniques+for+kidney+transplant+recipients%3A+a+systematic+review%5BTitle%5D)+AND+%22European+Urology%22%5BJournal%5D)">Minimally invasive, laparoscopic, and robotic-assisted techniques versus open techniques for kidney transplant recipients: a systematic review.</a> European Urology. 72 (2), 205-217 (2017).</li>
<li>Gastrich, M. D., Barone, J., Bachmann, G., Anderson, M., Balica, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+surgery%3A+review+of+the+latest+advances%2C+risks%2C+and+outcomes%5BTitle%5D)+AND+%22Journal+of+Robotic+Surgery%22%5BJournal%5D)">Robotic surgery: review of the latest advances, risks, and outcomes.</a> Journal of Robotic Surgery. 5 (2), 79-97 (2011).</li>
<li>Modi, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+assisted+kidney+transplantation%5BTitle%5D)+AND+%22Indian+Journal+of+Urology%22%5BJournal%5D)">Robotic assisted kidney transplantation.</a> Indian Journal of Urology. 30 (3), 287-292 (2014).</li>
<li>Vignolini, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+University+of+Florence+technique+for+robot-assisted+kidney+transplantation%3A+3-year+experience%5BTitle%5D)+AND+%22Frontiers+in+Surgery%22%5BJournal%5D)">The University of Florence technique for robot-assisted kidney transplantation: 3-year experience.</a> Frontiers in Surgery. 7, 583798(2020).</li>
<li>Musquera, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robot-assisted+kidney+transplantation%3A+update+from+the+European+Robotic+Urology+Section+%28ERUS%29+series%5BTitle%5D)+AND+%22BJU+International%22%5BJournal%5D)">Robot-assisted kidney transplantation: update from the European Robotic Urology Section (ERUS) series.</a> BJU International. 127 (2), 222-228 (2021).</li>
<li>Breda, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robot-assisted+kidney+transplantation%3A+the+European+experience%5BTitle%5D)+AND+%22European+Urology%22%5BJournal%5D)">Robot-assisted kidney transplantation: the European experience.</a> European Urology. 73 (2), 273-281 (2018).</li>
<li>Siena, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robot-assisted+kidney+transplantation+with+regional+hypothermia+using+grafts+with+multiple+vessels+after+extracorporeal+vascular+reconstruction%3A+results+from+the+European+Association+of+Urology+Robotic+Urology+Section+Working+Group%5BTitle%5D)+AND+%22European+Urology+Focus%22%5BJournal%5D)">Robot-assisted kidney transplantation with regional hypothermia using grafts with multiple vessels after extracorporeal vascular reconstruction: results from the European Association of Urology Robotic Urology Section Working Group.</a> European Urology Focus. 4 (2), 175-184 (2018).</li>
<li>Prudhomme, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic-assisted+kidney+transplantation+in+obese+recipients+compared+to+non-obese+recipients%3A+the+European+experience%5BTitle%5D)+AND+%22World+Journal+of+Urology%22%5BJournal%5D)">Robotic-assisted kidney transplantation in obese recipients compared to non-obese recipients: the European experience.</a> World Journal of Urology. 39 (4), 1287-1298 (2020).</li>
<li>Vignolini, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Development+of+a+robot-assisted+kidney+transplantation+programme+from+deceased+donors+in+a+referral+academic+centre%3A+technical+nuances+and+preliminary+results%5BTitle%5D)+AND+%22BJU+International%22%5BJournal%5D)">Development of a robot-assisted kidney transplantation programme from deceased donors in a referral academic centre: technical nuances and preliminary results.</a> BJU International. 123 (3), 474-484 (2019).</li>
<li>Ahlawat, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robotic+kidney+transplantation+with+regional+hypothermia+versus+open+kidney+transplantation+for+patients+with+end+stage+renal+disease%3A+an+ideal+stage+2B+study%5BTitle%5D)+AND+%22Journal+of+Urology%22%5BJournal%5D)">Robotic kidney transplantation with regional hypothermia versus open kidney transplantation for patients with end stage renal disease: an ideal stage 2B study.</a> Journal of Urology. 205 (2), 595-602 (2021).</li>
</ol>

The authors have no conflicts of financial and non-financial interests to disclose.

Although laparoscopic and robotic-assisted techniques have been widely applied for living donor nephrectomy, kidney transplantations are still mainly performed using conventional open techniques. Recently, however, a minimally invasive approach for kidney transplantation has been increasingly used. Compared with traditional open surgery, minimally invasive kidney transplantation has a lower risk of surgical site infection, incisional hernia, and wound dehiscence, as well as shorter hospitalization12</su...

Kidney transplantation contributes to prolonged survival and a better quality of life compared with peritoneal dialysis or hemodialysis1. Although the open approach is the standard procedure for kidney transplantation, robotic-assisted techniques have been recently adopted2,3,4. Specifically, robot-assisted kidney transplantation (RAKT) has several advantages over open kidney transplantation: minimal postoperative pain, better cosmesis, fewer wound infections, and shorter hospital stay5.&#160;Moreover, minimally invasive access and robotic technology enable surgeons to safely perform kidney transplants in morbidly obese patients6,7,8,9. However, due to its complexity, RAKT requires a learning curve to achieve sufficient reproducibility in the operation time, functional results, and safety10.
Allografts with multiple vessels usually require vascular reconstruction, which leads to extended cold and warm ischemic times. Despite the technical challenges of RAKT, a European multicenter study reported that RAKT using allografts with multiple vessels is technically feasible and leads to favorable functional results11. Although it is more common to place the kidney allograft in the pelvis medially during vascular anastomosis, according to previous reports4,5,6,7,8,9, the allograft was placed on the peritoneal pouch lateral to the iliac vessels in this protocol. Although it may be safe to put an allograft medially during anastomosis and flip it to the peritoneal pouch, this technique may not be familiar for inexperienced surgeons. Furthermore, it is more convenient to perform vascular anastomosis with the allograft in the peritoneal pouch and renal vessels in the proper position. This paper describes the step-by-step procedures for RAKT without flipping.

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robot-assisted kidney transplantation

Robot-Assisted Kidney Transplantation

This paper describes robot-assisted kidney transplantation (RAKT) from a living donor. The robot is docked between the parted legs of the patient, placed in the supine Trendelenburg position. Kidney allografts are provided by a living donor. Before vascular anastomosis, the kidney allograft is prepared by inserting a double-J stent in the ureter, and the temperature for the anastomosis is lowered by wrapping it in an ice-packed gauze. A 12 mm or 8 mm port for the robotic camera and three 8 mm ports for robotic arms are placed. A peritoneal pouch is created for the kidney allograft by raising the peritoneal flaps on both sides over the psoas muscle before dissecting the iliac vessels and bladder. A 6 cm Pfannenstiel incision is made to insert the kidney into the peritoneal pouch, lateral to the right iliac vessels. 
After clamping the external iliac vein with Bulldogs clamps, a venotomy is performed, and the graft renal vein is anastomosed to the external iliac vein in an end-to-side continuous manner with a 6/0 polytetrafluoroethylene suture. After clamping the graft renal vein, the iliac vein is declamped. This is followed by clamping of the external iliac artery, arteriotomy, arterial anastomosis with a 6/0 polytetrafluoroethylene suture, clamping of the graft renal artery, and declamping of the external iliac artery. Reperfusion is then carried out, and ureteroneocystostomy is performed using the Lich-Gregoir technique. The peritoneum is closed at a few locations with polymer locking clips, and a closed-suction drain is placed through one of the working ports. After deflating the pneumoperitoneum, all incisions are closed.

We set up a routine clinical pathway for recipients who have RAKT at our center. Renal Doppler ultrasound is performed one day post-transplant and technetium-99m diethylenetriamine penta-acetic acid renal scan two days post-transplant. For venous thromboembolism prophylaxis, an intermittent pneumatic compression device is applied during the first 24 h after RAKT. Foley catheter is removed on the fourth postoperative day. On the fifth day, a closed-suction drain is removed after confirming no intra-abdominal complication ...

This paper provides technical details for robot-assisted kidney transplantation from a living donor.

This paper describes robot-assisted kidney transplantation (RAKT) from a living donor. The robot is docked between the parted legs of the patient, placed ...

Research

JoVE Journal

Medicine

Renal transplantation in mice is a technically challenging procedure that requires careful post-operative care and treatment for success.

Murine Renal Transplantation Procedure

Here, we present a protocol to study the immunology of rejection. The surgical model presented reports a short operating time and a concise technique. Depending on the donor-recipient strain combination, the transplanted kidney may develop acute cellular rejection or chronic allograft damage, defined by interstitial fibrosis and tubular atrophy.

Mouse Kidney Transplantation: Models of Allograft Rejection

The goal of this manuscript is to describe the steps required to perform a kidney transplant in a mouse, paying particular attention to the details of the arterial anastomosis.

Murine Kidney Transplant Technique

Porcine models of organ transplantation provide an important platform to study mechanisms of organ preservation. This article describes a heterotopic porcine renal autotransplantation model, which allows investigating new approaches to improve the outcome of transplantation using marginal kidney grafts.

Heterotopic Renal Autotransplantation in a Porcine Model: A Step-by-Step Protocol

The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Large animal models play an essential role in preclinical transplantation research. Due to its similarities to the clinical setup, the porcine model of orthotopic kidney auto-transplantation described in this article provides an excellent in vivo setting for the testing of organ preservation techniques and therapeutic interventions.

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

This protocol presents a new surgical technique of mouse kidney transplantation focusing on a modified arterial anastomosis strategy. A vascular suture technique including a simple and safer ureter-bladder anastomosis method is also presented. These modifications shorten the operation time and improve the success rate of the mouse kidney transplantation procedure.

A Modified Surgical Technique for Kidney Transplantation in Mice

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes.  Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to  transplantation.

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

Learn robot-assisted kidney transplantation using a lateral allograft positioning technique that avoids flipping. This protocol demonstrates vascular anastomosis with reduced risk of vessel torsion for improved surgical outcomes.

Robot-Assisted Kidney Transplantation

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