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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

DOI:
10.3791/62234-v

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09:39 min

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February 07, 2021

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, , ,
1Department of Materials Science & Engineering,Stanford University, 2Department of Chemical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Department of Pediatrics – Endocrinology,Stanford University

Chapters

  • 00:04Introduction
  • 00:49Nanoparticle (NP) Synthesis
  • 02:10Hydrogel Formulation and Rheological Property Measurement
  • 03:27In Vitro Drug Release Characterization
  • 04:35Thermal Stability of Gel-Encapsulated Insulin Characterization
  • 05:19Cell Viability Assessment
  • 06:08Cell Settling Assessment
  • 07:15Results: Representative Polymer Nanoparticle (PNP) Hydrogel Release Kinetics, Cargo Stability, and Cell Viability
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the synthesis and formulation of injectable, supramolecular polymer-nanoparticle (PNP) hydrogel biomaterials. Applications of these materials for drug delivery, biopharmaceutical stabilization, and cell encapsulation and delivery are demonstrated.

Tags

InjectableSupramolecularPolymer-nanoparticleHydrogelsCell DeliveryDrug DeliveryNano-precipitationPEG-PLADynamic Light ScatteringHPMC-C12Rheological PropertiesDrug Release

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