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Journal
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Biochemistry
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使用沉降机进行冷冻电镜和单颗粒分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Cryo-EM and Single-Particle Analysis with Scipion
Please note that all translations are automatically generated.
Click here for the English version.
使用沉降机进行冷冻电镜和单颗粒分析
DOI:
10.3791/62261-v
•
09:06 min
•
May 29, 2021
•
A. Jiménez-Moreno
,
L. del Caño
,
M. Martínez
,
E. Ramírez-Aportela
,
A. Cuervo
,
R. Melero
,
R. Sánchez-García
,
D. Strelak
2,3
,
E. Fernández-Giménez
,
F.P. de Isidro-Gómez
,
D. Herreros
,
P. Conesa
,
Y. Fonseca
,
D. Maluenda
,
J. Jiménez de la Morena
,
J.R. Macías
,
P. Losana
,
R. Marabini
,
J.M. Carazo
,
C.O.S. Sorzano
4
1
Centro Nacional de Biotecnología
,
Campus Universidad Autónoma de Madrid
,
2
Faculty of Informatics
,
Masaryk University
,
3
Institute of Computer Science
,
Masaryk University
,
4
Campus Urbanización Montepríncipe
,
Universidad San Pablo CEU
Chapters
00:05
Introduction
00:53
Project Creation and Data Import
01:42
Movie Alignment: From Movies to Micrographs
02:37
Particle Picking
05:28
Evaluation and Post-Processing
06:53
Results: Representative Single-Particle Analysis
08:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
冷冻电子显微镜中的单颗粒分析是用于在高分辨率下确定生物集合体结构的主要技术之一。Scipion提供了创建整个管道的工具,以处理显微镜获取的信息并实现生物标本的3D重建。
Tags
Cryo-EM
Single-particle Analysis
Scipion
Image Processing
Workflow
Interoperability
Traceability
Reproducibility
Cryo-electron Tomography
Atomic Modeling
Movie Alignment
Optical Flow
Particle Picking
Micrograph
Power Spectral Density
Trajectory
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