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Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

DOI:
10.3791/62266-v

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12:16 min

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July 24, 2021

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, , ,
1School of Life Sciences,Westlake University, 2Westlake Laboratory of Life Sciences and Biomedicine, 3Institute of Basic Medical Sciences,Westlake Institute for Advanced Study, 4School of Basic Medical Sciences,Xi’an JiaoTong University, 5Office of Public Affairs,Westlake University, 6School of Pharmaceutical Sciences,Jilin University

Chapters

  • 00:00Introduction
  • 00:32Implant Preparation
  • 02:02Surgery
  • 09:22Virus Injection
  • 10:17In Vivo Imaging
  • 10:46Results
  • 11:37Conclusion

Summary

Automatic Translation

Automatic Translation

This article demonstrates the preparation of a custom-made imaging window supplemented with infusion cannula and its implantation onto the CA1 region of the hippocampus in mice.

Tags

Keywords: CraniotomyHippocampusNeuronal ActivitiesIn Vivo ImagingMouseSolderingAdhesiveStereotaxic FrameSurgeryAnesthesiaFur RemovalSkin Incision

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