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Immunology and Infection

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JoVE Journal Immunology and Infection
Производство человеческих CRISPR-инженерных CAR-T-клеток
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Производство человеческих CRISPR-инженерных CAR-T-клеток

Article DOI: 10.3791/62299-v • 06:33 min • March 15th, 2021
March 15th, 2021 •
*1,2, *1,2, 1,2, 1,2
1Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, 2Parker Institute for Cancer Immunotherapy, University of Pennsylvania
* These authors contributed equally

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Здесь мы представляем протокол редактирования генов в первичных Т-клетках человека с использованием технологии CRISPR Cas для модификации CAR-T-клеток.

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Иммунология и инфекция выпуск 169 Химерный антигенный рецептор (CAR)-Т-клетки клеточная иммунотерапия генная терапия лентивирусный вектор редактирование генов CRISPR/Cas9 Т-лимфоциты
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