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Journal / Developmental Biology / A Microfluidics Approach for the Functional…
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Developmental Biology

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JoVE Journal Developmental Biology
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
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A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Article DOI: 10.3791/62318-v • 08:06 min • March 19th, 2021
March 19th, 2021 •
*1, *1, 1
1Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht
* These authors contributed equally

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Summary
Automatic Translation

A protocol for the culture and manipulation of mouse embryonic tissue on a microfluidic chip is provided. By applying pulses of pathway modulators, this system can be used to externally control signaling oscillations for the functional investigation of mouse somitogenesis.

Tags

Microfluidics Approach Functional Investigation Signaling Oscillations Somitogenesis Dynamic Signaling Pathways Modulating Dynamics Microfluidic System Mouse Embryos Tissue Cultures Organoid Cultures PDMS Preparation Mixing Ratio Polymerization Chip Mold Curing Process Inlet And Outlet Holes Glass Slide Bonding
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