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Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models
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Biology
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JoVE Journal Biology
Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

DOI:
10.3791/62330-v

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07:49 min

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March 23, 2021

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, , , , , , ,
1STEMCELL Technologies Ltd., 2STEMCELL Technologies Inc., 3Terry Fox Laboratory, BC Cancer

Chapters

  • 00:04Introduction
  • 00:32Inversion of Intestinal Organoid Polarity
  • 02:57Dissociating Intestinal Organoids for Monolayer Generation and Culture
  • 05:21Results I: Analysis and Characterisation of the Morphology and Cell Polarity of Intestinal Organoids
  • 06:27Results II: Analysis and Characterisation of the Morphology and Differentiation Markers of Intestinal Monolayers
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present two protocols that allow the modeling of intestinal apical-specific interactions. Organoid-derived intestinal monolayers and Air-Liquid Interface (ALI) cultures facilitate the generation of well-differentiated epithelia accessible from both luminal and basolateral sides, whereas polarity-inverted intestinal organoids expose their apical side and are amenable to high throughput assays.

Tags

Keywords: Intestinal OrganoidApical-specificOrientation-specificMonolayersHigh-throughput ScreeningDissociationAnti-adherenceDMEM F-12Intestinal Organoid Expansion MediumTrypsin-EDTA

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