Presented here is a protocol for preserving the vascular contractility of PCLS murine lung tissue, resulting in a sophisticated three-dimensional image of the pulmonary vasculature and airway, which can be preserved for up to 10 days that is susceptible to numerous procedures.
Klouda, T., Kim, H., Kim, J., Visner, G., Yuan, K. Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies. J. Vis. Exp. (171), e62392, doi:10.3791/62392 (2021).