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Chapters
- 00:04Introduction
- 00:49Extraction of Mouse Lung
- 03:34Precision Cut Lung Slices (PCLS)
- 05:35Vasoconstrictor Experiment
- 06:20Preparing the Tissue for RNA or Protein Lysis on PCLS
- 07:26Results: Ex Vivo Pulmonary Vessel Structure and Contractility Studies of PCLS
- 08:32Conclusion
Presented here is a protocol for preserving the vascular contractility of PCLS murine lung tissue, resulting in a sophisticated three-dimensional image of the pulmonary vasculature and airway, which can be preserved for up to 10 days that is susceptible to numerous procedures.
