Journal
/
Biology
/
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

DOI:
10.3791/62396-v

•

10:02 min

•

May 27, 2021

•

, ,
1Department of Physics,Virginia Tech, 2Center for Soft Matter and Biological Physics,Virginia Tech

Chapters

  • 00:04Introduction
  • 01:14Preparation of Lipid Suspension for Extrusion
  • 02:24Extrusion of Hydrated Lipid Suspension
  • 04:44Reduction of The Collected Data
  • 08:11Results: Analysis of Liposomal Suspensions with Different Deuteration Schemes
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes the protocols for sample preparation, data reduction, and data analysis in neutron spin echo (NSE) studies of lipid membranes. Judicious deuterium labeling of lipids enables access to different membrane dynamics on mesoscopic length and time scales, over which vital biological processes occur.

Tags

Keywords: Neutron Spin Echo SpectroscopyLipid Membrane DynamicsMembrane-protein InteractionsIsotope SensitivityNanoscaleMembrane PropertiesCell PathologiesLipid VesiclesSample PreparationData ReductionHydrationFreeze-thaw CyclesExtrusion

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62396?article_type=v

The Microfluidic Probe: Operation and Use for Localized Surface Processing
check_url/62396?article_type=v

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
check_url/62396?article_type=v

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
check_url/62396?article_type=v

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
check_url/62396?article_type=v

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
check_url/62396?article_type=v

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
check_url/62396?article_type=v

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
check_url/62396?article_type=v

Imaging FITC-dextran as a Reporter for Regulated Exocytosis
check_url/62396?article_type=v

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
check_url/62396?article_type=v

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Read Article