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Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells
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JoVE Journal Medicine
Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

DOI:
10.3791/62435-v

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10:05 min

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April 12, 2021

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, ,
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-sen University, Guangzhou, China, 510060

Chapters

  • 00:04Introduction
  • 01:19Human Pluripotent Stem Cells (hPSCs) Culture
  • 03:48Embryoid Body (EB) Formation
  • 05:56Seed the Embryoid Bodies (EBs)
  • 07:00Detach and Culture OVs and RPE in Suspension to Obtain Retinal Organoids (ROs)
  • 07:45Results: Analysis of Morphological Features of ROs and Subtypes of Retinal Cells from hPSCs
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe an optimized retinal organoid induction system, which is suitable for various human pluripotent stem cell lines to generate retinal tissues with high reproducibility and efficiency.

Tags

Keywords: Retinal OrganoidHuman Pluripotent Stem CellsRetinal InductionRetinal DevelopmentDisease ModelingCell TherapyRetinal Degenerative DiseasesEmbryoid BodyPlating DensityRetinal CellsEDTA Dissociation

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