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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
 

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Article DOI: 10.3791/62438-v
February 12th, 2022

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Summary February 12th, 2022

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.

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