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小鼠肝细胞类器官的建立与遗传操作
JoVE Journal
Biology
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JoVE Journal Biology
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
DOI:

14:54 min

February 12, 2022

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Chapters

  • 00:01Introduction
  • 00:47Murine Hepatocyte Isolation by Liver Perfusion and Digestion
  • 02:47Hepatocyte Organoids Culture
  • 04:29Single Cells from Hepatocyte Organoids
  • 07:22siRNA or Plasmid Transfection
  • 12:58Representative Results
  • 13:58Conclusion

Summary

Automatic Translation

从小鼠肝细胞建立了长期 的体外 3D类器官培养系统。这些类器官可以通过shRNA/异位构建的慢病毒感染,siRNA转染和CRISPR-Cas9工程进行遗传和遗传操纵。

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