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Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
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Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Article DOI: 10.3791/62447-v • 09:19 min • March 28th, 2021
March 28th, 2021 •
1, 2, 1, 1, 2, 1
1Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, 2Department of Medicine, Naomi Berrie Diabetes Center, Columbia University

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Summary
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We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.

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Nanoparticle Tracking Analysis NTA Quantification Size Determination Extracellular Vesicles Operator Bias NTA Parameters Standardized Method Rigor Reproducibility Cuvette Statistically Random Sample Serial Dilution Ideal Dilution Factor Procedure Kungheng Cai Anthony Ferrante's Laboratory Nanoparticle Tracking Analysis Workspace Lint-free Material Gloves Magnetic Cuvette Jig Insert Pipette Purified Extracellular Vesicles Mixing Sample Air Bubbles Particle Concentration
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