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Journal
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Developmental Biology
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一种从人类多能干细胞中生成肾类器官的简化方法
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种从人类多能干细胞中生成肾类器官的简化方法
DOI:
10.3791/62452-v
•
07:39 min
•
April 13, 2021
•
Aneta Przepiorski
,
Amanda E. Crunk
,
Teresa M. Holm
,
Veronika Sander
,
Alan J. Davidson
,
Neil A. Hukriede
3
1
Department of Developmental Biology
,
University of Pittsburgh, School of Medicine
,
2
Department of Molecular Medicine and Pathology, School of Medical Sciences
,
University of Auckland
,
3
Center for Critical Care Nephrology
,
University of Pittsburgh, School of Medicine
Chapters
00:04
Introduction
00:59
Setting up the Kidney Organoid Assay and Feeding by Half-Medium Change
02:49
Transfer of Embryoid Bodies to Stage II Medium
04:49
Transfer to Spinner Flask and Feeding
05:37
Setting up a 6-Well Magnetic Stir Plate
06:14
Results: Immunofluorescence of the Kidney Organoids
06:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一种从人类多能干细胞(hPSCs)生成肾类器官的方案。该方案在两周内产生肾脏类器官。所得的类肾类器官可以在大型旋转瓶或多孔磁力搅拌板中培养,以进行平行的药物测试方法。
Tags
Kidney Organoids
Human Pluripotent Stem Cells
HPSCs
Cell Culture
Renal Development
Renal Disease
Simplified Method
Cell Differentiation
Embryoid Bodies
Cell Culture Techniques
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