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Immunology and Infection

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JoVE Journal Immunology and Infection
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Article DOI: 10.3791/62486-v • 10:25 min • June 5th, 2021
June 5th, 2021 •
1,2, 1,2, 1,2, 1, 1,2, 1,2, 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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Summary
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The protocol described here outlines a fast and effective method for measuring neutralizing antibodies against the SARS-CoV-2 spike protein by evaluating the ability of convalescent serum samples to inhibit infection by an enhanced green fluorescent protein-labeled vesicular stomatitis virus pseudotyped with spike glycoprotein.

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Detection SARS-CoV-2 Neutralizing Antibodies High-throughput Fluorescent Imaging Pseudovirus Infection Vaccines Neutralizing Antibody Response Containment Level Two Facility Fast Inexpensive Analyzing Samples Culturing Vero E6 Cells DMEM FBS Trypsin EDTA Incubation
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