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The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
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Biochemistry
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JoVE Journal Biochemistry
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

DOI:
10.3791/62491-v

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06:50 min

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June 05, 2021

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, , , , , , , , , ,
1European Molecular Biology Laboratory, 2ALPX S.A.S., 3Institut de Biologie Structurale

Chapters

  • 00:04Introduction
  • 00:32Crystallization Screening Experiments
  • 02:42Crystal Optimization Experiments
  • 03:18Crystal Harvesting and Tracking
  • 04:21Synchrotron X-Ray Diffraction Experiments
  • 05:27Results
  • 06:08Conclusion

Summary

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Here, we describe how to use the automated macromolecular crystallography pipelines for protein-to-structure, rapid ligand-protein complex analysis and large-scale fragment screening based on the CrystalDirect technology at the HTX Laboratory in EMBL Grenoble.

Tags

Keywords: Automated CrystallographyEMBL HTX FacilityGrenobleCrystalDirect TechnologyCRIM SoftwareCrystal Graphic Information Management SystemCrystallization ProtocolCrystallization ScreensHigh Throughput Crystallization LabAutomated ImagersCRIMSCrystallization PlatesCrystallization ExperimentsCrystal HarvestingProtein Structure DeterminationFragment ScreeningLigand Screening

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