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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

DOI:
10.3791/62494-v

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08:15 min

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September 08, 2021

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, , ,
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Chapters

  • 00:04Introduction
  • 00:44Bone Marrow Collection and Fixation
  • 01:30Embedding Bone Marrow in Agarose
  • 02:11Embedding Bone Marrow in Resin
  • 03:38Ultrathin Sectioning
  • 04:24Toluidine Blue Staining
  • 04:54Heavy Metal Staining
  • 05:31Transmission Electron Microscopy (TEM)
  • 06:02Results: In Situ Ultrastructure Exploration of Murine Megakaryopoiesis
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.

Tags

Keywords: MegakaryocytesBone MarrowTransmission Electron MicroscopyIn Situ ExplorationMurineMaturation StagesUltrastructureFixationEmbeddingResin EmbeddingDehydrationStaining

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