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Isolation of Mouse Megakaryocyte Progenitors

Isolation of Mouse Megakaryocyte Progenitors

Article DOI: 10.3791/62498-v 10:30 min May 20th, 2021
May 20th, 2021



This method describes the purification by flow cytometry of MEP and MKp from mice femurs, tibias, and pelvic bones.



Isolation Mouse Megakaryocyte Progenitors Lineage Hierarchy Cell Culture Molecular Assays Single Cell Level Ethical Practice Cell Sorting MEP MKP Populations Bone Collection C57 Black Six Mouse 70% Ethanol Incision Skin Dissection Pad Pelvic Bones Iliac Crest Lumbar Region Hind Limbs Muscles Vertebrae Tail Forceps Scalpels
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