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Isolation of Mouse Megakaryocyte Progenitors
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JoVE Journal Biology
Isolation of Mouse Megakaryocyte Progenitors

Isolation of Mouse Megakaryocyte Progenitors

DOI:
10.3791/62498-v

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10:30 min

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May 20, 2021

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, , , ,
1Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, 2UMR-S1113 –IRFAC, Université de Strasbourg

Chapters

  • 00:04Introduction
  • 00:38Mouse Bone Collection
  • 03:10Magnetic Depletion of Lineage Positive Cells
  • 07:17Results: Purification of MouseMEP and MKp from Femurs, Tibias, and Pelvic Bones by Flow Cytometry
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes the purification by flow cytometry of MEP and MKp from mice femurs, tibias, and pelvic bones.

Tags

MouseMegakaryocyteProgenitorsCell SortingMEPMKPBoneC57 Black SixIliac CrestFemurTibiaPBSNBCSDissectionCell Isolation

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