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August 26, 2021
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It is now acknowledged that the three-dimensional environment of cells can play an important role in their behavior, maturation and/or differentiation. This protocol describes a three-dimensional cell culture model designed to study the impact of physical containment and mechanical constraints on megakaryocytes.
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Boscher, J., Gachet, C., Lanza, F., Léon, C. Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement. J. Vis. Exp. (174), e62511, doi:10.3791/62511 (2021).
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