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Megakaryocyt Kultur i 3D Methylcellulose-baserede Hydrogel at forbedre cellemodning og studere virkningen af stivhed og indespærring
JoVE Journal
Biology
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JoVE Journal Biology
Megakaryocyte Culture in 3D Methylcellulose-Based Hydrogel to Improve Cell Maturation and Study the Impact of Stiffness and Confinement
DOI:

07:53 min

August 26, 2021

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Chapters

  • 00:05Introduction
  • 00:53Cell Embedding in Methylcellulose Hydrogel
  • 03:03Cell Resuspension for Proplatelet Analysis
  • 04:24Cell Fixation and Retrieval for Future Analyses
  • 05:16Results: Megakaryocyte Characteristics and Proplatelet Quantification According to Culture Conditions
  • 07:08Conclusion

Summary

Automatic Translation

Det er nu anerkendt, at de tre-dimensionelle miljø af celler kan spille en vigtig rolle i deres adfærd, modning og / eller differentiering. Denne protokol beskriver en tredimensionel cellekulturmodel designet til at studere virkningen af fysisk indeslutning og mekaniske begrænsninger på megakaryocytter.

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