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JoVE Journal
Biochemistry
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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
 

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Article DOI: 10.3791/62512
May 8th, 2021

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Summary May 8th, 2021

Here, we present an optimized on-filter digestion protocol with detailed information about the following: protein digestion, peptide purification and data independent acquisition analysis. This strategy is applied to the analysis of expressed prostatic secretions-urine samples and allows high proteome coverage and low missing value label-free profiling of the urinary proteome.

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