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Journal / Biochemistry / Proteomisk profil av EPS-urin genom…
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Biochemistry
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JoVE Journal Biochemistry
Proteomisk profil av EPS-urin genom FASP-matsmältning och dataoberoende analys
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Proteomisk profil av EPS-urin genom FASP-matsmältning och dataoberoende analys

Article DOI: 10.3791/62512-v • 14:48 min • May 8th, 2021
May 8th, 2021 •
1, 1, 2, 3, 3, 1, 4, 1
1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 2Department of Health Science, Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro

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Här presenterar vi ett optimerat matsmältningsprotokoll på filter med detaljerad information om följande: proteinsammanfattning, peptidrening och dataoberoende förvärvsanalys. Denna strategi tillämpas på analys av uttryckt prostata sekret-urin prover och tillåter hög proteome täckning och lågt saknade värde etikett-fri profilering av urinary proteome.

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Biokemi nummer 171
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