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Journal
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Biochemistry
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使用FlashPack方法进行简单的内部超高性能毛细管柱制造
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal
Biochemistry
Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Please note that all translations are automatically generated.
Click here for the English version.
使用FlashPack方法进行简单的内部超高性能毛细管柱制造
DOI:
10.3791/62522-v
•
13:36 min
•
December 04, 2021
•
Sergey I. Kovalchuk
,
Rustam Ziganshin
,
Irina Shelukhina
1
Laboratory of Bioinformatics Methods in Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
,
Russian Academy of Sciences
,
2
Department of Molecular Neuroimmune Signalling, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
,
Russian Academy of Sciences
Chapters
00:00
Introduction
01:34
Protocol Introduction
02:06
Stage 1:Packing Station Assembly
02:26
Stage 2: Capillary Preparation
02:59
Stage 3: Sorbent Slurry Preparation
04:45
Stage 4: Capillary Packing in a Pressure Bomb
09:09
Stage 5: Packing in the HPLC System
11:35
Representative Results
12:59
Conclusions
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个优化的FlashPack毛细管柱包装程序的协议。将优化的实验方案应用于常见的 100 bar 压力弹设置,可将长超高性能毛细管柱的包装和制造速度提高 10 倍。
Tags
In-house Capillary Column Manufacturing
FlashPack Approach
Ultra-high Performance Capillary Liquid Chromatography
LC-MS Proteomics
Peptide Separation
Capillary Columns
Sorbent Packing
High Sorbent Slurry Concentration
Pressure Bomb Packing
Magnet Bar
Packing Rate
Ultra-high Performance Sorbents
Long Columns
Column Preparation Procedure
Packing Station
Nitrogen Gas Tank
Pressure Bomb
Magnetic Stirrer
PEEK Capillary
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