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Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
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Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Article DOI: 10.3791/62534-v • 09:48 min • April 22nd, 2022
April 22nd, 2022 •
*1,2, *3, *1, 1,4
1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University
* These authors contributed equally

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Summary
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Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants.

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H3K4me3 Modification Profiling Plants Cleavage Under Targets And Tagmentation Chromatin Protein-affiliated DNA Fragments Intact Nuclei Isolation Chromatin Modifications Recognition Antibody Tethering Protein A-transposase Fusion Protein Tn5 Transposase Magnesium Ions Activation Adapters Integration DNA Preparation Polymerase Chain Reaction Enrichment Epigenomics Regulation Histone Modifications Transcription Factors Behaviors Non-coding RNAs With Recruited Proteins Temporal And Spatial Control Of Gene Expression CUT&Tag Technology Henikoff Lab Enzyme Tethering Method Chromatin Epigenomics Profiling Cotton Leaves As Experimental Materials
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