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Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord
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Neuroscience
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JoVE Journal Neuroscience
Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

DOI:
10.3791/62537-v

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09:01 min

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May 23, 2021

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, , , ,
1Department of Pharmacology & Physiology,Drexel University College of Medicine

Chapters

  • 00:04Introduction
  • 00:57Uptake of sEVs by Neuro-2a Cells
  • 01:38Primary Astrocytic Cultures
  • 03:34Immunofluorescence
  • 05:03In Vivo Uptake of sEVs and Immunohistochemistry
  • 06:43Results: Uptake of Fluorescent Labeled Small Extracellular Vesicles
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to label macrophage-derived small extracellular vesicles with PKH dyes and observe their uptake in vitro and in the spinal cord after intrathecal delivery.

Tags

Keywords: Fluorescent Labeled Small Extracellular VesiclesSEVs UptakeSpinal CordIn VitroCell VisualizationNeuro-2a CellsPostnatal PupsCortical LobesDissociationAstrocytesMicrogliaOligodendrocytesMechanistic StudiesTherapeutic StudiesSpinal DisordersPainInflammation

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