A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Surgery,University Medical Center Schleswig-Holstein, 2Institute of Medical and Marine Biotechnology,University of Lübeck, 3Fraunhofer Research and Development Center for Marine and Cellular Biotechnology, 4Institute for Surgical Pathology, Medical Center – University of Freiburg, Faculty of Medicine,University of Freiburg, 5Tumorbank Comprehensive Cancer Center Freiburg,Medical Center – University of Freiburg, 6Core Facility Histopathology and Digital Pathology Freiburg,Medical Center – University of Freiburg
Chapters
- 00:00Introduction
- 00:54Preparation of Low-Melting Agarose and Vibratome
- 01:58Tissue Embedding in Low-Melting Agarose
- 03:13Slicing of the Agarose-Embedded Tissue Using a Vibratome
- 03:51Culture of Organotypic Slice Cultures
- 04:38Formalin Fixation and Paraffin Embedding of OTSCs
- 06:20Hematoxylin and Eosin Staining
- 07:09Results: Morphology, Viability, and Histopathological Evaluation of the OTSCs at 6 and 15 Days of Primary PDAC and Metastasis
- 08:50Conclusion
Questo protocollo descrive la preparazione di colture organotipiche di fette (OTSC). Questa tecnica facilita la coltivazione ex vivo di tessuto multicellulare intatto. Gli OTSC possono essere utilizzati immediatamente per testare la rispettiva risposta ai farmaci in un ambiente multicellulare.
