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Injections of AAV Vectors for Optogenetics in Anesthetized and Awake Behaving Non-Human Primate Brain
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Chapters
- 00:04Introduction
- 00:51Making a Cannula
- 02:09Loading the Vector Solution for Injection
- 04:14Cannula Insertion into the Target Brain Area and Injection
- 06:33Results: Optogenetic Manipulations by Adeno-Associated Viral (AAV) Vector Injections in Awake Monkeys
- 08:39Conclusion
As currently implemented, optogenetics in non-human primates requires injection of viral vectors into the brain. An optimal injection method should be reliable and, for many applications, capable of targeting individual sites of arbitrary depth that are readily and unambiguously identified in postmortem histology. An injection method with these properties is presented.
