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Journal / Biochemistry / Capturing Actively Produced Microbial Volatile…
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Biochemistry

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JoVE Journal Biochemistry
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Article DOI: 10.3791/62547-v • 09:19 min • June 1st, 2022
June 1st, 2022 •
1, 1, 2, 3, 3, 3, 1
1Department of Molecular Biology and Biochemistry, University of California Irvine, 2Department of Ecology and Evolutionary Biology, University of California Irvine, 3Entech Instruments Inc.

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Summary
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This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing.

Tags

Microbial Volatile Organic Compounds Vacuum-assisted Sorbent Extraction VASE Volatile Metabolites Volatile Analysis Clinical Samples Metabolic Biomarker Discovery Disease States Fecal Samples BHI Medium 13C Glucose Deuterium Sewage Samples Technical Triplicates Saliva Samples
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