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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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Summary
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In the present protocol, we demonstrate how to visualize DNA double-strand end resection during S/G2 phase of the cell cycle using an immunofluorescence-based method.

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Global DNA Double-strand End Resection BrdU-DNA Labeling Cell Cycle Discrimination Imaging Quantification Of DNA And Resection Cell Cycle Marking Bromodeoxyuridine Signal Jean-Yves Masson Sofiane Y.Mersaoui Sterile Culture Hood Six-well Plate HeLa Cells Transfection Drug Treatment BrdU Incubation X-ray Irradiation Pre-extraction Buffer A Cytoskeleton Stripping Buffer B Fixation
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