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Vurdering av global DNA Double-Strand End Resection ved hjelp av BrdU-DNA-merking kombinert med cell cycle diskriminering imaging
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Vurdering av global DNA Double-Strand End Resection ved hjelp av BrdU-DNA-merking kombinert med cell cycle diskriminering imaging

Article DOI: 10.3791/62553-v • 06:44 min • April 28th, 2021
April 28th, 2021 •
*1,2, *1,2, 2,3, 1,2
1Oncology Division, Genome Stability Laboratory, CHU de Québec Research Center, HDQ Pavilion, 2Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University Cancer Research Center, 3Oncology Division, CHU de Québec Research Center, CHUL Pavilion
* These authors contributed equally

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I den nåværende protokollen demonstrerer vi hvordan man visualiserer DNA dobbeltstrenget end reseksjon under S / G2-fasen av cellesyklusen ved hjelp av en immunfluorescence-basert metode.

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Biologi utgave 170 BrdU-foci immunfluorescens DNA-endereseksjon homolog rekombinasjon PARP-1 PARP-hemmere PARP-hemmermotstand BMN673
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