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Assessing Respiratory Immune Responses to Haemophilus Influenzae
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing Respiratory Immune Responses to Haemophilus Influenzae

Assessing Respiratory Immune Responses to Haemophilus Influenzae

DOI:
10.3791/62572-v

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06:32 min

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June 29, 2021

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, , , , ,
1Monash Lung and Sleep,Monash Medical Centre, 2Monash University Department of Medicine,Monash Medical Centre, 3Flow Cytometry Science Technology Platform,Francis Crick Institute, 4Clinical Immunology,Monash Medical Centre, 5Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute,Monash University

Chapters

  • 00:04Introduction
  • 00:38Assessment of Phagocytic Function by Flow Cytometry
  • 01:17Assessment of Lymphocyte Function in Peripheral Blood
  • 02:30Assessment of Lymphocyte Function/ Inflammatory Mediators in Lung Tissue
  • 04:16Assessment of Lung Proteolysis by Confocal Microscopy
  • 04:45Results: ROS and Cytokine Production by Monocytes and In Situ Zymography of the Lung Tissue
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

Haemophilus influenzae induces inflammation in the respiratory tract. This article will focus on the use of flow cytometry and confocal microscopy to define immune responses by phagocytes and lymphocytes in response to this bacterium.

Tags

Keywords: Haemophilus InfluenzaeLung InflammationCOPDPneumoniaInnate Immune ResponseAdaptive Immune ResponseFlow CytometryPeripheral BloodAntigen StimulationCytokine AnalysisInfection Assay

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