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Genetics

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JoVE Journal Genetics
Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes
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Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes

Article DOI: 10.3791/62588-v • 07:45 min • May 1st, 2021
May 1st, 2021 •
1, 2, 1, 1,3, 1,2
1Department of Microbiology & Molecular Genetics, University of California, Irvine, 2Department of Molecular Biology & Biochemistry, University of California, Irvine, 3Vector Biology Department, Liverpool School of Tropical Medicine

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Summary
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The protocols reported here illustrate three alternative ways to assess the performance of genetically-engineered mosquitoes destined for vector control in laboratory-contained small cage trials. Each protocol is tailored to the specific modification the mosquito strain bears (gene drive or non-gene drive) and the types of parameters measured.

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Small-cage Laboratory Trials Genetically-engineered Anopheline Mosquitoes Transgenic Mosquito Performance Wild Type Counterparts Malaria Elimination Experimental Designs Threat Assessment Trans-gene Introduction Initial Phase Cages Setup Larval Addition Blood Meal Source Oviposition Container Egg Hatching Selection Of Larvae Control Cages Release Ratio Pupae Addition
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