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Lille plasmamembranpræparat til analyse af Candida albicans Cdr1-mGFPHis
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Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Lille plasmamembranpræparat til analyse af Candida albicans Cdr1-mGFPHis

DOI:
10.3791/62592-v

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09:44 min

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June 13, 2021

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, , , , ,
1Sir John Walsh Research Institute, Faculty of Dentistry,University of Otago, 2Department of Microbiology, Faculty of Medicine,Chulalongkorn University, 3School of Biological Sciences,University of Auckland

Chapters

  • 00:04Introduction
  • 00:57Growing and Harvesting Yeast Cells
  • 02:26Isolation of Plasma Membranes
  • 04:14Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
  • 06:22Results: Analysis of Cdr1-mGFPHis Isolated from Plasma Membranes of Saccharomyces cerevisiae
  • 09:06Conclusion

Summary

Automatic Translation

Automatic Translation

Denne artikel præsenterer en lille plasmamembran isolation protokol for karakterisering af Candida albicans ABC (ATP-bindende kassette) protein Cdr1, overekspresseret i Saccharomyces cerevisiae. En protease-cleavable C-terminal mGFPHis dobbelt tag med en 16-rest linker mellem Cdr1 og mærket var designet til at lette rensning og vaskemiddel-screening af Cdr1.

Tags

Keywords: Plasma MembraneCandida AlbicansCdr1-mGFPHisMembrane ProteinSmall-scale IsolationATPaseDetergentCell HarvestingPre-cultureCell LysisCentrifugationHomogenization Buffer

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