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Lille plasmamembranpræparat til analyse af Candida albicans Cdr1-mGFPHis
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Lille plasmamembranpræparat til analyse af Candida albicans Cdr1-mGFPHis

Article DOI: 10.3791/62592-v • 09:44 min • June 13th, 2021
June 13th, 2021 •
*1, *1, 1, 1,2, 3, 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland
* These authors contributed equally

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Denne artikel præsenterer en lille plasmamembran isolation protokol for karakterisering af Candida albicans ABC (ATP-bindende kassette) protein Cdr1, overekspresseret i Saccharomyces cerevisiae. En protease-cleavable C-terminal mGFPHis dobbelt tag med en 16-rest linker mellem Cdr1 og mærket var designet til at lette rensning og vaskemiddel-screening af Cdr1.

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Biologi udgave 172
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