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Маломасштабная плазматическая мембранная подготовка для анализа Candida albicans Cdr1-mGFPHis
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Маломасштабная плазматическая мембранная подготовка для анализа Candida albicans Cdr1-mGFPHis

Article DOI: 10.3791/62592-v • 09:44 min • June 13th, 2021
June 13th, 2021 •
*1, *1, 1, 1,2, 3, 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland
* These authors contributed equally

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В данной статье представлен маломасштабный протокол изоляции плазматической мембраны для характеристики белка Candida albicans ABC (АТФ-связывающая кассета) Cdr1, сверхэкспрессированного в Saccharomyces cerevisiae. Расщепленная протеаза C-концевая двойная метка mGFPHis с 16-остатчным линкером между Cdr1 и меткой была разработана для облегчения очистки и моющего скрининга Cdr1.

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Биология выпуск 172
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