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1Allergy Unit,Hospital Regional Universitario de Málaga, 2Allergy Research Group,Instituto de Investigación Biomédica de Málaga-IBIMA, 3Universidad de Málaga, Departamento de Biología celular, Genética y Fisiología,Universidad de Málaga, 4Departamento de Medicina,Universidad de Málaga, 5Nanostructures for Diagnosing and Treatment of Allergic Diseases Laboratory,Andalusian Center for Nanomedicine and Biotechnology-BIONAND
Chapters
- 00:05Introduction
- 01:33Sample and Staining Mix Preparation
- 02:57Blood Stimulation
- 03:52Erythrocytes Lysing
- 04:46Flow Cytometry Acquisition
- 05:35Results: Basophil Activation Test
- 06:39Conclusion
The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry.
