In Vitro Allergy Test
In Vitro Allergy Test - Related Videos
07:22
Basophil Activation Test for Allergy Diagnosis
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Cited by 9 •
2021
The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry.
04:34
Mouse Body Temperature Measurement Using Infrared Thermometer During Passive Systemic Anaphylaxis and Food Allergy Evaluation
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Cited by 44 •
2018
Here we present a new method to accurately measure body temperature differences in passive systemic anaphylaxis (PSA) and food allergy mouse models using an infrared thermometer. This procedure has been accurately duplicated in previous PSA results.
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
10:13
Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
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Cited by 10 •
2015
We have developed an eye irritation test which utilizes a three dimensional reconstructed human cornea-like epithelial (RhCE) tissue model. The test is able to discriminate between ocular irritant and corrosive materials (GHS Categories 1 and 2 combined) and those that do not require labeling (GHS No Category).
FAQs
The assay exposes a patient’s serum to selected allergen extracts or molecular components. If allergen-specific immunoglobulin E is present, it binds to the relevant allergen and is captured for measurement. Enzyme-based or fluorescence-based detection systems then indicate the amount of bound IgE, allowing the laboratory to identify immune sensitization to particular targets.
The tested material determines which immune targets the assay can evaluate. Allergen extracts represent selected allergen sources, while molecular components allow testing against specified parts of those sources. Clinicians choose the relevant targets according to the suspected allergy, such as food, drug, respiratory, or insect-sting exposure, rather than testing an undefined collection of substances.
A positive result demonstrates immune sensitization, meaning that allergen-specific IgE was detected in the sample. It does not, by itself, establish that exposure caused the patient’s symptoms or determine their clinical significance. Clinicians therefore interpret the result together with symptom patterns and medical history before using it to support diagnosis or management.
An In Vitro Allergy Test examines a biological sample instead of producing a skin reaction. This makes it an alternative or complement when skin testing is unsuitable or could pose risks. The laboratory result and skin-based findings are not interpreted in isolation; each contributes evidence that must be considered with the patient’s symptoms and history.
A laboratory workflow begins with collecting a patient’s blood or another appropriate biological sample, commonly using serum for the assay. The sample is exposed to selected allergen extracts or molecular components, and any allergen-specific IgE is captured. Enzyme- or fluorescence-based detection then measures the captured antibodies and produces a result for clinical interpretation.
Clinicians may select this approach when skin testing is unsuitable or could present risks for the patient. It can also complement skin-based evaluation by providing laboratory evidence from a blood or other biological sample. The choice depends on the suspected allergy and the need to assess sensitization to relevant food, drug, respiratory, or insect-sting allergens.
These assays can support evaluation of suspected food, drug, respiratory, and insect-sting allergies. Testing is directed toward allergen extracts or molecular components relevant to the clinical question. Results do not replace assessment of symptoms and medical history, but they can add objective evidence of sensitization and help clinicians develop an appropriate diagnostic and management plan.
The result should be interpreted as one part of a broader clinical assessment. Detected allergen-specific IgE indicates sensitization to the tested target, while the patient’s symptoms and medical history help determine whether that sensitization is clinically relevant. This combined interpretation supports diagnostic decisions and can guide subsequent allergy management.