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半翅目肠道中植物病毒和昆虫载体蛋白的免疫荧光标记

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Biology

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JoVE Journal Biology

Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts

Please note that all translations are automatically generated. Click here for the English version.

半翅目肠道中植物病毒和昆虫载体蛋白的免疫荧光标记

DOI:

10.3791/62605-v

•

07:16 min

•

May 14, 2021

•

Lu Zhang, Wenwen Liu, Xifeng Wang

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences

Chapters

00:04Introduction
00:42Virus Acquisition, Collection of Viruliferous Insects, and Reagent Preparation
01:24Dissection of Adults and Excision of Guts
02:38Dissection of Nymphs and Excision of Guts
03:47Labeling Protocols for SRBSDV Virions and an Insect Protein
05:33Results: Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
06:32Conclusion

Summary

Automatic Translation

这种用于切除昆虫肠道中植物病毒蛋白和载体昆虫蛋白的免疫荧光标记的协议可用于研究病毒和载体昆虫之间的相互作用、昆虫蛋白功能和病毒传播的分子机制。

Tags

Keywords: Immunofluorescent Labeling Plant Virus Insect Vector Hemipteran Gut Laser Scanning Confocal Microscopy Southern Rice Black Streaked Dwarf Virus (SRBSDV)Gut Dissection PBS Paraformaldehyde Triton X-100 Insect Paralysis Gut Extraction

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