Journal
/
Cancer Research
/
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

DOI:
10.3791/62622-v

•

11:51 min

•

September 22, 2021

•

, , , , ,
1Cell Therapy Facility,Moffitt Cancer Center and Research Institute, 2Department of Immunology,Moffitt Cancer Center and Research Institute

Chapters

  • 00:04Introduction
  • 01:29Lymphocyte Isolation and Alpha-beta (αβ) T Cell Depletion
  • 06:16Co-culture with Artificial Antigen-Presenting Cells (aAPCs) and Cell Harvest
  • 09:22Results: Characterization and Optimization of the γδ T Cell Expansion Process
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

Presented is a protocol for the expansion of Gamma-Delta (γδ) T cell drug product. Lymphocytes are isolated by elutriation and γδ-enriched with zoledronic acid and interleukin-2. Alpha-beta T cells are depleted using a clinical-grade magnetic separation device. The γδ cells are co-cultured with K562-derived, artificial antigen-presenting cells and expanded.

Tags

Keywords: Gamma-delta T CellsCytotoxic ActivityCancerAcute Myeloid LeukemiaExpansionEnrichmentApheresisCell TherapyElutriationCell CultureBioreactorZoledronic AcidIL-2Flow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62622?article_type=v

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
check_url/62622?article_type=v

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma – An Improved Protocol
check_url/62622?article_type=v

Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
check_url/62622?article_type=v

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
check_url/62622?article_type=v

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
check_url/62622?article_type=v

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment
check_url/62622?article_type=v

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
check_url/62622?article_type=v

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
check_url/62622?article_type=v

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
check_url/62622?article_type=v

Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures

Read Article