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Journal / Cancer Research / En automatiserad mikroskopisk poängsättningsmetod …
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Cancer Research
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JoVE Journal Cancer Research
En automatiserad mikroskopisk poängsättningsmetod för γ-H2AX Foci-analys hos humana perifera blodlymfocyter
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En automatiserad mikroskopisk poängsättningsmetod för γ-H2AX Foci-analys hos humana perifera blodlymfocyter

Article DOI: 10.3791/62623-v • 08:23 min • December 25th, 2021
December 25th, 2021 •
1, 2, 1, 3, 1, 1, 1, 1, 4, 5, 6, 1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH

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Detta protokoll presenterar bildberedning och automatiserad poängsättning av γ-H2AX foci assay på perifera blod lymfocyter. För att illustrera analysens metod och känslighet bestrålades isolerade lymfocyter in vitro. Denna automatiserade metod för DNA DSB-detektion är användbar för snabba och höggenomströmningsbiologiska dosimetriapplikationer.

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Cancerforskning nummer 178
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