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Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

DOI:
10.3791/62626-v

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07:15 min

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November 22, 2021

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, , , , , , ,
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology,Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital,Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences

Chapters

  • 00:04Introduction
  • 00:40Pseudovirus Packaging with Calcium‐Phosphate Transfection
  • 01:33Detection of the HA, NA, and HIV‐1 P24 Protein Expression of Influenza Pseudovirus
  • 02:24Pseudovirus Titration
  • 03:22Luciferase Assay
  • 04:06Pseudovirus Neutralization (PN) Assay
  • 05:16Results: HPAI H5 Pseudovirus Preparations and Pseudovirus Neutralization Assays
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.

Tags

Keywords: Pseudo-typed H5 Avian Influenza VirusesCalcium Phosphate TransfectionAntibody Neutralizing ActivityHemagglutinin Protein ExpressionPseudo Virus TitrationMDCK CellsLuciferase Assay

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