Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

This protocol helps to guide the selection of regions of interest for spatial omics technologies, which allows a more targeted characterization of tissues or cell populations. An automated protocol for ROI selection in proteomics assays is more robust and reproducible than manual protocols, and using 50 micrometer ROIs for transcriptomics assays allows profiling of specific cell populations. Start with programming the autostainer to apply fluorescent visualization antibodies.

In the autostainer software, click on the home button and choose Protocols. Then click on Create/Edit Protocols and select RUO Discovery Universal as the procedure. Click on First Sequence, then select Deparaffinazation, followed by Depar v2.